Renal protective foods with functional food bioactives against age-associated decline in renal function (Hill's geriatric Beagle colony)
Eighty-one healthy geriatric Beagles (mean 10.4 years) at the Hill's Pet Nutrition colony were randomly assigned, after blocking for sex, to a control renal protective food or to one of two functional foods (FF1, FF2) with increasing amounts of fish oil, lipoic acid, fruits and vegetables and higher quality protein, for 6 months; 30 mature adult dogs served as a cross-sectional comparison. GFR, lean body percent and circulating biomarkers were measured. GFR increased over time in all three geriatric groups, FF2 lowered SDMA by 14.3%, and serum protein rose; the authors conclude functional food bioactives can temporarily reverse the age-associated decline in renal function.
Record
| Design | randomized controlled trial |
|---|---|
| Status | completed |
| Setting | laboratory colony |
| Intervention | Control renal protective food supplemented with increasing amounts of functional food bioactives (fish oil, lipoic acid, fruits and vegetables, higher quality protein sources): functional foods FF1 and FF2 |
| Intervention class | diet or supplement |
| Comparator | Traditional renal protective food (control; energy dense and mildly protein-restricted; Hill's Science Diet Mature Adult) |
| Dose regimen | Both functional foods contained 0.5% fish oil and 100 mg/kg lipoic acid; FF1 contained 2.75% fruits and vegetables, 7% egg protein and 7.5% wet meat chicken; FF2 contained 7.5% fruits and vegetables, 14% egg protein, 10% wet meat chicken and 955 IU/kg a-tocopherol acetate |
| Duration | 6 months |
| Dogs | 111 |
| Population note | 81 geriatric dogs randomly assigned to three foods (n = 27 per group) after blocking for sex, plus 30 mature-adult dogs as a cross-sectional comparison group |
| Breed | Beagle |
| Age | geriatric: mean age 10.4, range 7.9-14.2 years; mature adult: mean age 5.0, range 3.3-6.9 years |
| Other | healthy colony dogs; 35 ovariohysterectomized females, 44 neutered males and 2 intact males among the geriatric dogs |
| Primary outcome | Glomerular filtration rate (GFR), lean body percent (LB%) by dual energy x-ray absorptiometry, and circulating biomarkers and metabolites including symmetric dimethylarginine (SDMA) |
| Result (as reported) | Geriatric dogs consuming all three foods increased (P<0.001) GFR over time; group averages ranged from 13.0-16.9%. Dogs fed the highest supplemented level of bioactives (FF2) had lower (P<0.001) SDMA concentrations (-14.3%). Feeding functional foods did not alter body weight, but increased (P<0.001) serum protein concentration (+6.7%). The authors conclude that supplementation with functional food bioactives can temporarily reverse the age-associated decline in renal function and serum total protein. |
| Lead organisation | Pet Nutrition Center, Hill's Pet Nutrition, Inc., Topeka, KS |
| Sponsor or funder | Hill's Pet Nutrition, Inc. |
| Tags | diet, supplement, renal, sdma, gfr, fish-oil, lipoic-acid, colony |
Notes: Separate from the client-owned test-food study (PMID 27088214), which used different dogs, setting and comparator.
Sources and verbatim quotes
Nutritional Interventions that Slow the Age-Associated Decline in Renal Function in a Canine Geriatric Model for Elderly Humans. (2016) PMID 27925141 (results)
To determine the effects of feeding traditional and renal protective foods (RPF) supplemented with functional food bioactives on glomerular filtration rate (GFR), lean body percent (LB%), and selected circulating biomarker and metabolite concentrations in a geriatric dog model.
Randomized block design and cross-sectional study.
Hill's Pet Nutrition, Inc. dog colony.
Eighty-one geriatric dogs (mean age, 10.4; range, 7.9-14.2 years) and 30 mature-adult dogs (mean age, 5.0; range, 3.3-6.9 years).
Geriatric dogs were fed one of three foods (n = 27 per group) for 6 months: a traditional RPF (control) that was energy dense and mildly protein-restricted, or control food supplemented with increasing amounts of functional food bioactives: fish oil, lipoic acid, fruits and vegetables, and higher quality protein sources [functional foods one (FF1) and two (FF2)].
Renal function was assessed by GFR, LB% was determined by dual energy x-ray absorptiometry, and circulating biomarkers and metabolites were measured in blood.
Geriatric dogs consuming all three foods increased (P<0.001) GFR over time; group averages ranged from 13.0-16.9%.
Dogs fed the highest supplemented level of bioactives (FF2) had lower (P<0.001) symmetric dimethylarginine (SDMA) concentrations (-14.3%).
Feeding functional foods did not alter body weight, but increased (P<0.001) serum protein concentration (+6.7%).
Supplementation with functional food bioactives can temporarily reverse the age-associated decline in renal function and serum total protein.
Nutritional Interventions that Slow the Age-Associated Decline in Renal Function in a Canine Geriatric Model for Elderly Humans. (2016) PMID 27925141 (design)
The study protocol was reviewed and approved by the Institutional Animal Care and Use Committee, Hill's Pet Nutrition, Inc., Topeka, KS, USA (Permit Number: CP354).
This was a feeding trial involving 81 healthy, geriatric Beagle dogs (35 ovariohysterectomized females, 44 neutered males, and 2 intact males) with mean age of 10.4 years (range: 7.9 to 14.2 years) and mean initial BW of 14.1 kg (range: 9.0 to 18.1 kg).
After blocking for sex, dogs were randomly assigned to one of three food groups (n=27 each; control, FF1, and FF2; Table 1).
The control food was Hill's Science Diet Mature Adult, Hill's Pet Nutrition, Inc.
The two functional foods were designed to increase the healthy lifespan of aging dogs.
Both functional foods contained 0.5% fish oil, and 100 mg/kg lipoic acid.
FF1 contained unique protein sources compared with control food in that egg protein (7%), corn gluten meal (6.4%), and wet meat chicken (7.5%) replaced other carbohydrate and protein sources in control food.
FF2 differed from control and FF1 in that it contained additional amounts of fruits and vegetables; i.e. beet pulp, citrus pulp, carrot granules, dried spinach, and tomato pomace (each present at 1.50% of formula; total 7.5%), more a-tocopherol acetate (955 IU/kg, as fed; Table 1), and increased egg protein (14%) and wet meat chicken (10%), but no corn gluten meal.
Funded by and performed at the Pet Nutrition Center, Hill's Pet Nutrition, Inc., Topeka, KS.
Reproduce: canine_trial_search(trial_id="hills-functional-foods-renal-function-geriatric-colony-2016") in dog-geroscience-mcp; dataset canine-trial-registry.