Dietary astaxanthin and age-associated mitochondrial dysfunction in young and geriatric Beagles
Healthy female Beagles in a young (about 3 years) and a geriatric (about 10.7 years) group, 14 per age group, were fed 0 or 20 mg astaxanthin daily for 16 weeks to examine modulation of age-associated mitochondrial dysfunction. Leukocyte mitochondrial function and plasma oxidative markers were measured at weeks 0, 8 and 16. Astaxanthin increased ATP production, mitochondrial mass and cytochrome c oxidoreductase activity, especially in geriatric dogs, and lowered nitric oxide in both age groups.
Record
| Design | controlled trial |
|---|---|
| Status | completed |
| Intervention | Astaxanthin fed daily |
| Intervention class | diet or supplement |
| Comparator | 0 mg astaxanthin |
| Dose regimen | 0 or 20 mg astaxanthin daily for 16 wk |
| Duration | 16 wk (blood sampled wk 0, 8 and 16) |
| Dogs | 28 |
| Population note | n=14 per age group (young and geriatric), fed 0 or 20 mg astaxanthin; group sizes per treatment within age group not stated |
| Breed | Beagle |
| Age | young (2.97±0.01 yr) and geriatric (10.71±0.01 yr) |
| Other | healthy female |
| Primary outcome | Leukocyte mitochondrial function (membrane permeability, ATP production, cytochrome c oxidase/reductase, mitochondrial number) and plasma oxidative stress markers |
| Result (as reported) | Mitochondrial function improved in both young and geriatric dogs by increasing (P<0.05) ATP production, mitochondria mass, and cytochrome c oxidoreductase activity, especially in geriatric dogs compared with young dogs; astaxanthin also increased the reduced to oxidized glutathione ratio in young dogs and decreased nitric oxide in both age groups. Dietary astaxanthin improved mitochondrial function in blood leukocytes, most likely by alleviating oxidative damage to cellular DNA and protein. |
| Tags | supplement, astaxanthin, mitochondria, oxidative-stress, beagle |
Notes: The abstract does not describe randomization or blinding; the PubMed publication type for this record lists Randomized Controlled Trial. No full text available in the corpus; the total n of 28 is 14 per age group across two age groups.
Sources and verbatim quotes
Astaxanthin modulates age-associated mitochondrial dysfunction in healthy dogs. (2013) PMID 23100599 (results)
Young (2.97±0.01 yr; 8.16±0.15 kg BW) and geriatric (10.71±0.01 yr; 9.46±0.18 kg BW) healthy female Beagle dogs (n=14/age group) were fed 0 or 20 mg astaxanthin daily for 16 wk to examine modulation of mitochondrial function.
Fasted blood was sampled on wk 0, 8, and 16.
Mitochondria membrane permeability, ATP production, cytochrome c oxidase/reductase, and number were assessed in leukocytes whereas astaxanthin uptake, glutathione, superoxide dismutase, nitric oxide, 8-hydroxy-2'-deoxyguanosine, 8-isoprostane, and protein carbonyl were measured in plasma.
Mitochondrial function improved in both young and geriatric dogs by increasing (P<0.05) ATP production, mitochondria mass, and cytochrome c oxidoreductase activity, especially in geriatric dogs compared with young dogs.
Astaxanthin feeding also increased (P<0.05) the reduced glutathione to oxidized glutathione ratio in young dogs and decreased (P<0.05) nitric oxide in both young and geriatric dogs.
Dietary astaxanthin improved mitochondrial function in blood leukocytes, most likely by alleviating oxidative damage to cellular DNA and protein.
Reproduce: canine_trial_search(trial_id="astaxanthin-mitochondrial-function-beagles-2013") in dog-geroscience-mcp; dataset canine-trial-registry.